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Quick Answer: Tirzepatide and metformin together provide complementary diabetes control by targeting different metabolic pathwaysmetformin reduces hepatic glucose production while tirzepatide enhances insulin secretion and promotes weight loss

For protein extraction, each tissue was placed into a tube containing a lysis buffer consisting of 20 mM Tris HCl with pH 7.6, 0.5 M Sucrose, 0.15 M KCl, 20 mM DTT supplemented with 100 L/mL of a protease inhibitor cocktail (Sigma-Aldrich, MO, USA) in a 1:3 (v/v) ratio

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