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BPC 157 (10 ng/kg, 10 g/kg i.p.), L-NAME (5 mg/kg i.p.), and L-arginine (200 mg/kg i.p.) were given alone or together at (i) 30 min before or, alternatively, (ii) at 5 min after isoprenaline (75 or 150 mg/kg s.c.)

Subsequent studies indicated that the two peptides (P11 and P12) with the stapling to i , i + 7 position derived from tryptophan 13 (Trp13) to Glu33 of autophagy related 16 like 1 gene (ATG16L1) showed high binding affinity to ATG5 ( K d = 36 nmol/L) compared to the control linear peptide (P13) with the K d of 0.072 mol/L, which could be partially given the credit to the stabilization of the helical by stapling [135]

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