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the column was washed with 20 ml equilibration solution, and elution was performed with a linear salt gradient from 0 to 250 mM NaCl (20 mM di-sodium hydrogen phosphate di-hydrate, pH 7.5) followed by 40 ml of isocratic elution with 1 M NaCl (20 mM di-sodium hydrogen phosphate di-hydrate, pH 7.5)

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