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Although, like other SLC22 family members, SLC22A4 is capable of transporting multiple substrates, the transport efficiencies for other confirmed substrates are orders of magnitude lower than that of ergothioneine, and increasing evidence from independent laboratories demonstrates SLC22A4 is highly specific for ergothioneine (28,31,32)
10.1111/j.1471-4159.2006.04393.x 69
Improving early identification of serious conditions remains a priority across primary care, which is why the new national pathway for catching red flags earlier is attracting attention
Because Cr GPDH expression did not impact proliferation of HeLa cells and robustly decreased both DHAP and Gro3P levels and the NADH/NAD + ratio, we used the Cr GPDH construct in the rest of the experiments in this study
Future studies will reveal the applicability of our model, for example, to activate enzymatically inactive glutaredoxins, to alter the sensitivity and responsiveness of glutaredoxin-coupled fluorescent redox sensors, to further address the catalytic mechanism with GSSCys, HEDS and physiological substrates, or to design specific transition-state inhibitors
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